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NEK3 Antibody

Product Specifications

Background

In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. NEK3 is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm.

NCBI Gene ID

4752

Swiss Prot

P51956

Accession Number

P51956

Host

Rabbit

Reactivity

Human

Immunogen

This NEK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the Central region of human NEK3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

58 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

NEK3

NCBI Official Name

Serine/threonine-protein kinase Nek3

NCBI Organism

Homo sapiens

Background Reference 01

Schultz, S.J., et al., Cell Growth Differ. 5 (6) :625-635 (1994) .

Background Reference 02

Schultz, S.J., et al., Cell Growth Differ. 4 (10) :821-830 (1993) .

Background Reference 03

Kimura, M., et al., Cytogenet. Cell Genet. 95 (3-4), 177-182 (2001) .

Other Product Names

Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, NEK3

Tested Applications

WB

Protein ID

20178297

Physical Properties

Liquid

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