PLK3 Antibody

Product Specifications

Background

CNK, a member of the CDC5/Polo subfamily of Ser/Thr protein kinases, is involved in regulating M phase functions during the cell cycle. It may also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. This membrane-associated protein binds to the calcium/integrin-binding protein (CIB) . This interaction probably occurs via the POLO-box domain. Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. This protein exhibits a short half-live. In cells of hematopoietic origin, CNK is strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. Cytokine and cellular adhesion trigger CNK induction. CNK is thought to be phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis The protein contains 2 POLO box domains.

NCBI Gene ID

1263

Swiss Prot

Q9H4B4

Accession Number

Q9H4B4

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This PLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-646 amino acids from the C-terminal region of human PLK3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

72 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PLK3

NCBI Official Name

Serine/threonine-protein kinase PLK3

NCBI Organism

Homo sapiens

Background Reference 01

Holtrich, U., et al., Oncogene 19 (42) :4832-4839 (2000) .

Background Reference 02

Ouyang, B., et al., Oncogene 18 (44) :6029-6036 (1999) .

Background Reference 03

Ouyang, B., et al., J. Biol. Chem. 272 (45) :28646-28651 (1997) .

Background Reference 04

Li, B., et al., J. Biol. Chem. 271 (32) :19402-19408 (1996) .

Other Product Names

Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, FGF-inducible kinase, Polo-like kinase 3, PLK-3, Proliferation-related kinase, PLK3, CNK, FNK, PRK