ABL1 Antibody

Product Specifications

Background

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t (9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

NCBI Gene ID

Swiss Prot

P00519

Accession Number

P00519

Host

Rabbit

Reactivity

Human

Immunogen

This ABL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the N-terminal region of human ABL1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

123 kDa

Fragment

Rabbit IgG

Applications Notes

For WB starting dilution is: 1:1000

Symbol

ABL1

NCBI Official Name

Tyrosine-protein kinase ABL1

NCBI Organism

Homo sapiens

Background Reference 01

Donaldson, L.W., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (22) :14053-14058 (2002) .

Background Reference 02

Pisabarro, M.T., et al., J. Mol. Biol. 281 (3) :513-521 (1998) .

Background Reference 03

Nam, H.J., et al., Structure 4 (9) :1105-1114 (1996) .

Background Reference 04

Chissoe, S.L., et al., Genomics 27 (1) :67-82 (1995) .

Other Product Names

Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150, ABL1, ABL, JTK7