EGFR Antibody

Product Specifications

Background

EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

NCBI Gene ID

1956

Swiss Prot

P00533

Accession Number

P00533

Host

Rabbit

Reactivity

Human

Immunogen

This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 847-876 amino acids from human EGFR.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

134 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

EGFR

NCBI Official Name

Epidermal growth factor receptor

NCBI Organism

Homo sapiens

Background Reference 01

Aifa, S., et al., Exp. Cell Res. 302 (1) :108-114 (2005) .

Background Reference 02

Adams, T.E., et al., Growth Factors 22 (2) :89-95 (2004) .

Background Reference 03

Ichinose, J., et al., Biochem. Biophys. Res. Commun. 324 (3) :1143-1149 (2004) .

Background Reference 04

Kuribayashi, A., et al., Endocrinology 145 (11) :4976-4984 (2004) .

Other Product Names

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

Tested Applications

Protein ID

2811086

Physical Properties

Liquid

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