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GCAT Antibody

Product Specifications

Background

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

NCBI Gene ID

23464

Swiss Prot

O75600

Accession Number

O75600

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=62-951

Calculated Molecular Weight

45 kDa

Fragment

Rabbit Ig

Symbol

GCAT

NCBI Official Name

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

NCBI Organism

Homo sapiens

Background Reference 01

Edgar, A.J., Polak, J.M.Eur. J. Biochem. 267 (6), 1805-1812 (2000)

Background Reference 02

Tressel, T., Thompson, R., J. Biol. Chem. 261 (35), 16428-16437 (1986)

Other Product Names

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL

Tested Applications

WB, IHC-P, Flow

Physical Properties

Liquid

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