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Cdc25B Antibody

Product Specifications

Background

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

NCBI Gene ID

994

Swiss Prot

P30305

Accession Number

P30305

Host

Rabbit

Reactivity

Human

Immunogen

This Cdc25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human Cdc25B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=62-766

Calculated Molecular Weight

65 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

CDC25B

NCBI Official Name

M-phase inducer phosphatase 2

NCBI Organism

Homo sapiens

Background Reference 01

Uchida, S., et al., Biochem. Biophys. Res. Commun. 316 (1) :226-232 (2004) .

Background Reference 02

Ito, Y., et al., Int. J. Mol. Med. 13 (3) :431-435 (2004) .

Background Reference 03

Wu, W., et al., Cancer Res. 63 (19) :6195-6199 (2003) .

Background Reference 04

Mils, V., et al., Exp. Cell Res. 285 (1) :99-106 (2003) .

Other Product Names

M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2

Tested Applications

WB

Protein ID

21264471

Physical Properties

Liquid

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