SCYL1 Antibody

Product Specifications

Background

SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.

NCBI Gene ID

57410

Swiss Prot

Q96KG9

Accession Number

Q96KG9

Host

Rabbit

Reactivity

Human

Immunogen

This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

90 kDa

Fragment

Rabbit Ig

Symbol

SCYL1

NCBI Official Name

N-terminal kinase-like protein

NCBI Organism

Homo sapiens

Background Reference 01

Tang, Z., et al., Biochem. Biophys. Res. Commun. 324 (4) :1324-1332 (2004) .

Background Reference 02

Kato, M., et al., Genomics 79 (6) :760-767 (2002) .

Background Reference 03

Liu, S.C., et al., Biochim. Biophys. Acta 1517 (1) :148-152 (2000) .

Background Reference 04

Van Asseldonk, M., et al., Genomics 66 (1) :35-42 (2000) .

Other Product Names

N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase, SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP