AMPK alpha 1 Antibody

Product Specifications

Background

AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.

NCBI Gene ID

5562

Swiss Prot

Q13131

Accession Number

Q13131

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-510 amino acids from the C-terminal region of human AMPK alpha 1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Neuroscience, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

64 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:8000

Symbol

PRKAA1

NCBI Official Name

5'-AMP-activated protein kinase catalytic subunit alpha-1

NCBI Organism

Homo sapiens

Background Reference 01

Zhang, Q.H., et al., Genome Res. 10 (10) :1546-1560 (2000) .

Background Reference 02

Stapleton, D., et al., J. Biol. Chem. 271 (2) :611-614 (1996) .

Other Product Names

5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1