HSP27 Antibody

Product Specifications

Background

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.

NCBI Gene ID

3315

Swiss Prot

P04792

Accession Number

P04792

Host

Rabbit

Reactivity

Human

Immunogen

This HSP27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the Central region of human HSP27.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

23 kDa

Fragment

Rabbit Ig

Symbol

HSPB1

NCBI Official Name

Heat shock protein beta-1

NCBI Organism

Homo sapiens

Background Reference 01

Wano, C., et al., Exp. Cell Res. 298 (2) :584-592 (2004) .

Background Reference 02

Evgrafov, O.V., et al., Nat. Genet. 36 (6) :602-606 (2004) .

Background Reference 03

Song, H., et al., Biochem. Biophys. Res. Commun. 314 (1) :143-150 (2004) .

Background Reference 04

Chauhan, D., et al., Blood 102 (9) :3379-3386 (2003) .

Other Product Names

Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28