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Pyruvate Kinase Antibody

Product Specifications

Background

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

NCBI Gene ID

5315

Swiss Prot

P14618

Accession Number

P14618

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=62-705

Calculated Molecular Weight

58 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PKM

NCBI Official Name

Pyruvate kinase PKM

NCBI Organism

Homo sapiens

Background Reference 01

Lehner, B., et al., Genome Res. 14 (7) :1315-1323 (2004) .

Background Reference 02

Gevaert, K., et al., Nat. Biotechnol. 21 (5) :566-569 (2003) .

Background Reference 03

Valentini, G., et al., J. Biol. Chem. 277 (26) :23807-23814 (2002) .

Background Reference 04

Lowrie, D.J. Jr., et al., J. Struct. Biol. 132 (2) :83-94 (2000) .

Other Product Names

Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3, PKM2

Tested Applications

WB

Protein ID

20178296

Physical Properties

Liquid

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