PDXK Antibody
Product Specifications
Background
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.
NCBI Gene ID
8566
Swiss Prot
O00764
Accession Number
O00764
Host
Rabbit
Reactivity
Human
Immunogen
This PDXK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-272 amino acids from the C-terminal region of human PDXK.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Obesity, Signal Transduction
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
35 kDa
Fragment
Rabbit Ig
Symbol
PDXK
NCBI Official Name
Pyridoxal kinase
NCBI Organism
Homo sapiens
Background Reference 01
Shin, J.H., et al., Neurochem. Int. 45 (1) :73-79 (2004) .
Background Reference 02
Lee, H.S., et al., Mol. Cells 10 (4) :452-459 (2000) .
Background Reference 03
Laine-Cessac, P., et al., Biochem. Pharmacol. 54 (8) :863-870 (1997) .
Background Reference 04
Hanna, M.C., et al., J. Biol. Chem. 272 (16) :10756-10760 (1997) .
Other Product Names
Pyridoxal kinase, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK
Tested Applications
WB, IHC-P
Physical Properties
Liquid
Curated Selection
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