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NAGK Antibody

Product Specifications

Background

N-acetylglucosamine kinase (NAGK) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals. The predicted 344-amino acid NAGK protein contains the 5 sequence motifs necessary for the binding of ATP by sugar kinases. NAGK shares 91.6% amino acid similarity with mouse Nagk, for which enzyme activity is detectable in all mouse tissues examined, with highest enzymatic activity in testis. It is hypothesized that NAGK has a general role in the catabolic pathways of GlcNAc as well as of ManNAc.

NCBI Gene ID

55577

Swiss Prot

Q9UJ70

Accession Number

Q9UJ70

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This NAGK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human NAGK.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=62-643

Calculated Molecular Weight

37 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NAGK

NCBI Official Name

N-acetyl-D-glucosamine kinase

NCBI Organism

Homo sapiens

Background Reference 01

Hinderlich, S., et al., Eur. J. Biochem. 267 (11) :3301-3308 (2000) .

Background Reference 02

Lowes, W., et al., Biochim. Biophys. Acta 1379 (1) :134-142 (1998) .

Background Reference 03

Weidanz, J.A., et al., Br. J. Haematol. 95 (4) :645-653 (1996) .

Other Product Names

N-acetyl-D-glucosamine kinase, N-acetylglucosamine kinase, GlcNAc kinase, NAGK

Tested Applications

WB

Protein ID

24638065

Physical Properties

Liquid

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