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PRPS1/PRPS2/PRPS3 Antibody

Product Specifications

Background

Phosphoribosylpyrophosphate synthetase (PRPS; EC 2.7.6.1) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine, pyrimidine, and pyridine biosynthesis. By PCR of human lymphoblast mRNA using primers based on the cDNA sequence of rat PRS I (Prps1), Roessler et al. (1990) isolated a partial human PRPS1 cDNA. They used this partial cDNA to screen lymphoblast cDNA libraries and isolated additional cDNAs corresponding to the entire PRPS1 coding region. The deduced PRPS1 protein has 318 amino acids.

NCBI Gene ID

5631

Swiss Prot

P60891

Accession Number

P60891

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This PRPS1/PRPS2/PRPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the N-terminal region of human PRPS1/PRPS2/PRPS3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Obesity

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rat, Xenopus

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

35 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PRPS1

NCBI Official Name

Ribose-phosphate pyrophosphokinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Roessler, B. J., et al. Nucleic Acids Res. 18: 193 (1990) .

Other Product Names

Ribose-phosphate pyrophosphokinase 1, PPRibP, Phosphoribosyl pyrophosphate synthase I, PRS-I, PRPS1

Tested Applications

WB

Protein ID

46397477

Physical Properties

Liquid

Frequently Asked Questions

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