Aurora-A Antibody
Product Specifications
Background
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy.
NCBI Gene ID
6790
Swiss Prot
O14965
Accession Number
O14965
Host
Rabbit
Reactivity
Human
Immunogen
This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human Aurora-A.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Cell Cycle, Signal Transduction
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=62-580
Calculated Molecular Weight
46 kDa
Fragment
Rabbit Ig
Symbol
AURKA
NCBI Official Name
Aurora kinase A
NCBI Organism
Homo sapiens
Background Reference 01
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26) :16899-16903 (2002) . Tanaka, M., et al., J. Biol. Chem. 277 (12) :10719-10726 (2002) . Nigg, E.A., Nat. Rev. Mol. Cell Biol. 2 (1) :21-32 (2001) . Deloukas, P., et al., Nature 414 (6866) :865-871 (2001) . Shindo, M., et al., Biochem. Biophys. Res. Commun. 244 (1) :285-292 (1998) .
Other Product Names
Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, AURKA
Tested Applications
WB, IF, Flow
Physical Properties
Liquid
Curated Selection
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