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MLF1 Antibody

Product Specifications

Background

MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

NCBI Gene ID

4291

Swiss Prot

P58340

Accession Number

P58340

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human MLF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

31 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MLF1

NCBI Official Name

Myeloid leukemia factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Li, Z.F., J. Neurol. Sci. 264 (1-2), 77-86 (2008)

Background Reference 02

Yoneda-Kato, N., EMBO J. 24 (9), 1739-1749 (2005)

Other Product Names

Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1

Tested Applications

WB

Protein ID

17368170

Physical Properties

Liquid

Frequently Asked Questions

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