MMP25 Antibody

Product Specifications

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP25 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. In addition, it is thought that this protein is activated intracellularly by furin-like enzymes. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP20 but has been renamed matrix metalloproteinase 25 (MMP25) .

NCBI Gene ID

64386

Swiss Prot

Q9NPA2

Accession Number

Q9NPA2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This MMP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from the C-terminal region of human MMP25.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

63 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MMP25

NCBI Official Name

Matrix metalloproteinase-25

NCBI Organism

Homo sapiens

Background Reference 01

Matsuda, A., et al., J. Biol. Chem. 278 (38) :36350-36357 (2003) .

Background Reference 02

Velasco, G., et al., Cancer Res. 60 (4) :877-882 (2000) .

Background Reference 03

Nagase, H., et al., J. Biol. Chem. 274 (31) :21491-21494 (1999) .

Background Reference 04

Pei, D., Cell Res. 9 (4) :291-303 (1999) .

Other Product Names

Matrix metalloproteinase-25, MMP-25, 3424-, Leukolysin, Membrane-type matrix metalloproteinase 6, MT-MMP 6, MTMMP6, Membrane-type-6 matrix metalloproteinase, MT6-MMP, MT6MMP, MMP25, MMP20, MMPL1, MT6MMP