P-Glycoprotein Antibody

The membrane-associated ABCB1 protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White) . This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. ABCB1 is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

This P-Glycoprotein (ABCB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-677 amino acids from the Central region of human P-Glycoprotein (ABCB1) .

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Saito, S., et al., J. Hum. Genet. 47 (1) :38-50 (2002) . Kerb, R., et al., Pharmacogenomics 2 (1) :51-64 (2001) . Cascorbi, I., et al., Clin. Pharmacol. Ther. 69 (3) :169-174 (2001) . Hoffmeyer, S., et al., Proc. Natl. Acad. Sci. U.S.A. 97 (7) :3473-3478 (2000) . Mickley, L.A., et al., Blood 91 (5) :1749-1756 (1998) .