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PCNA Antibody

Product Specifications

Background

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

NCBI Gene ID

5111

Swiss Prot

P12004

Accession Number

P12004

Host

Rabbit

Reactivity

Human

Immunogen

This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PCNA.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Hamster, Monkey, Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=61-537

Calculated Molecular Weight

29 kDa

Fragment

Rabbit Ig

Symbol

PCNA

NCBI Official Name

Proliferating cell nuclear antigen

NCBI Organism

Homo sapiens

Background Reference 01

Wang, Y., J. Cell. Biochem. 106 (3), 409-413 (2009)

Background Reference 02

Maga, G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008)

Background Reference 03

Acharya, N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)

Other Product Names

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Tested Applications

WB, IHC-P, IF, Flow

Protein ID

129694

Physical Properties

Liquid

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