RFC5 Antibody
Product Specifications
Background
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
NCBI Gene ID
5985
Swiss Prot
P40937
Accession Number
P40937
Host
Rabbit
Reactivity
Human, Rat
Immunogen
This RFC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human RFC5.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=61-523
Calculated Molecular Weight
38 kDa
Fragment
Rabbit Ig
Symbol
RFC5
NCBI Official Name
Replication factor C subunit 5
NCBI Organism
Homo sapiens
Background Reference 01
Stelzl, U., Cell 122 (6), 957-968 (2005)
Background Reference 02
Andersen, J.S., Nature 433 (7021), 77-83 (2005)
Other Product Names
Replication factor C subunit 5, Activator 1 36 kDa subunit, A1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, RF-C 36 kDa subunit, RFC36, RFC5
Tested Applications
WB, Flow
Protein ID
728777
Physical Properties
Liquid
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
