SFRS2 Antibody

Product Specifications

Background

SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.

NCBI Gene ID

6427

Swiss Prot

Q01130

Accession Number

Q01130

Host

Rabbit

Reactivity

Human

Immunogen

This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human SFRS2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Pig, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

25 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

SRSF2

NCBI Official Name

Serine/arginine-rich splicing factor 2

NCBI Organism

Homo sapiens

Background Reference 01

Merdzhanova, G., Cell Death Differ. 15 (12), 1815-1823 (2008)

Background Reference 02

Solis, A.S., J. Biol. Chem. 283 (35), 23619-23626 (2008)

Background Reference 03

Donev, R., Mol. Psychiatry 12 (7), 681-690 (2007)

Background Reference 04

Sureau, A., Proc. Natl. Acad. Sci. U.S.A. 89 (24), 11683-11687 (1992)

Other Product Names

Serine/arginine-rich splicing factor 2, Protein PR264, Splicing component, 35 kDa, Splicing factor SC35, SC-35, Splicing factor, arginine/serine-rich 2, SRSF2, SFRS2