MARCKS Antibody

Product Specifications

Background

MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

NCBI Gene ID

4082

Swiss Prot

P29966

Accession Number

P29966

Host

Rabbit

Reactivity

Human

Immunogen

This MARCKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the C-terminal region of human MARCKS.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

32 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MARCKS

NCBI Official Name

Myristoylated alanine-rich C-kinase substrate

NCBI Organism

Homo sapiens

Background Reference 01

Rauch, M.E., et al., J. Biol. Chem. 277 (16) :14068-14076 (2002) .

Background Reference 02

Aderem, A., Biochem. Soc. Trans. 23 (3) :587-591 (1995) .

Background Reference 03

Rao, P.H., et al., Cytogenet. Cell Genet. 66 (4) :272-273 (1994) .

Background Reference 04

Taniguchi, H., et al., J. Biol. Chem. 268 (14) :9960-9963 (1993) .

Other Product Names

Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL