BMI1 Antibody

Product Specifications

Background

BMI is a component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2. BMI cooperates with the MYC oncogene to produce B-lymphomas.

NCBI Gene ID

100532731648

Swiss Prot

P35226

Accession Number

P35226

Host

Rabbit

Reactivity

Human

Immunogen

This BMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human BMI1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Chicken, Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

37 kDa

Fragment

Rabbit Ig

Symbol

BMI1

NCBI Official Name

Polycomb complex protein BMI-1

NCBI Organism

Homo sapiens

Background Reference 01

Smith, K.S., et al., Mol. Cell 12 (2) :393-400 (2003) . Xia, Z.B., et al., Proc. Natl. Acad. Sci. U.S.A. 100 (14) :8342-8347 (2003) . Itahana, K., et al., Mol. Cell. Biol. 23 (1) :389-401 (2003) . Dimri, G.P., et al., Cancer Res. 62 (16) :4736-4745 (2002) . Bardos, J.I., et al., J. Biol. Chem. 275 (37) :28785-28792 (2000) .