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HSF1 Sumoylation Site Antibody

Product Specifications

Background

Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.

NCBI Gene ID

3297

Swiss Prot

Q00613

Accession Number

Q00613

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This HSF1 Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-309 amino acids from human HSF1 Sumoylation Site.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=61-400

Calculated Molecular Weight

57 kDa

Fragment

Rabbit Ig

Symbol

HSF1

NCBI Official Name

Heat shock factor protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Hilgarth, et al., Biochem Biophys Res Commun. 2003 Mar 28;303 (1) :196-200.

Background Reference 02

He, H., et al., J. Biol. Chem. 278 (37) :35465-35475 (2003) .

Background Reference 03

Wang, X., et al., Mol. Cell. Biol. 23 (17) :6013-6026 (2003) .

Background Reference 04

Ignatenko, N.A., et al., Exp. Cell Res. 288 (1) :1-8 (2003) .

Other Product Names

Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1

Tested Applications

WB, IF

Protein ID

462333

Physical Properties

Liquid

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