WNK1 Antibody

Product Specifications

Background

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide- sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L.

NCBI Gene ID

65125

Swiss Prot

Q9H4A3

Accession Number

Q9H4A3

Host

Rabbit

Reactivity

Human

Immunogen

This WNK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1848-1882 amino acids from human WNK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

251 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

WNK1

NCBI Official Name

Serine/threonine-protein kinase WNK1

NCBI Organism

Homo sapiens

Background Reference 01

Verissimo F., et al.Oncogene 20:5562-5569 (2001) .

Background Reference 02

Vidal-Petiot E., et al.PLoS ONE 7:E37751-E37751 (2012) .

Background Reference 03

Scherer S.E., et al.Nature 440:346-351 (2006) .

Background Reference 04

Moore T.M., et al.J. Biol. Chem. 275:4311-4322 (2000) .

Other Product Names

Serine/threonine-protein kinase WNK1, 2.7.11.1, Erythrocyte 65 kDa protein, p65, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, hWNK1, WNK1, HSN2, KDP, KIAA0344, PRKWNK1