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NDC80 Antibody

Product Specifications

Background

Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

NCBI Gene ID

10403

Swiss Prot

O14777

Accession Number

O14777

Host

Rabbit

Reactivity

Human

Immunogen

This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-539 amino acids from human NDC80.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=61-289

Calculated Molecular Weight

74 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

NDC80

NCBI Official Name

Kinetochore protein NDC80 homolog

NCBI Organism

Homo sapiens

Background Reference 01

Chen Y., et al.Mol. Cell. Biol. 17:6049-6056 (1997) .

Background Reference 02

Chen Y., et al.J. Biol. Chem. 272:24081-24087 (1997) .

Background Reference 03

Zheng L., et al.Mol. Cell. Biol. 19:5417-5428 (1999) .

Background Reference 04

Zheng L., et al.Mol. Cell. Biol. 20:3529-3537 (2000) .

Other Product Names

Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2

Tested Applications

WB

Physical Properties

Liquid

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