EIF5 Antibody
Product Specifications
Background
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .
NCBI Gene ID
1983
Swiss Prot
P55010
Accession Number
P55010
Host
Rabbit
Reactivity
Human
Immunogen
This EIF5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 267-298 amino acids from the Central region of human EIF5.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
49 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:2000
Symbol
EIF5
NCBI Official Name
Eukaryotic translation initiation factor 5
NCBI Organism
Homo sapiens
Background Reference 01
Si K., et al.J. Biol. Chem. 271:16934-16938 (1996) .
Background Reference 02
Wiemann S., et al.Genome Res. 11:422-435 (2001) .
Background Reference 03
Ota T., et al.Nat. Genet. 36:40-45 (2004) .
Background Reference 04
Bechtel S., et al.BMC Genomics 8:399-399 (2007) .
Other Product Names
Eukaryotic translation initiation factor 5, eIF-5, EIF5
Tested Applications
WB
Protein ID
27735202
Physical Properties
Liquid
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