ACO1 Antibody

Product Specifications

Background

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.

NCBI Gene ID

Swiss Prot

P21399

Accession Number

P21399

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

This ACO1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 354-388 amino acids from the Central region of human ACO1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Chicken

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

98 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

ACO1

NCBI Official Name

Cytoplasmic aconitate hydratase

NCBI Organism

Homo sapiens

Background Reference 01

Hirling H., et al.Nucleic Acids Res. 20:33-39 (1992) .

Background Reference 02

Humphray S.J., et al.Nature 429:369-374 (2004) .

Background Reference 03

Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Background Reference 04

Rouault T.A., et al.Proc. Natl. Acad. Sci. U.S.A. 87:7958-7962 (1990) .

Other Product Names

Cytoplasmic aconitate hydratase, Aconitase, 4.2.1.3, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1, ACO1, IREB1