PPP2R4 Antibody

Product Specifications

Background

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A (i) ) in presence of ATP and Mg (2+) (By similarity) . Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A (D) in presence of ATP and Mg (2+) (in vitro) . The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A (D) :PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.

NCBI Gene ID

5524

Swiss Prot

Q15257

Accession Number

Q15257

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This PPP2R4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-35 amino acids from human PPP2R4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

41 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

PPP2R4

NCBI Official Name

Serine/threonine-protein phosphatase 2A activator

NCBI Organism

Homo sapiens

Background Reference 01

Cayla X., et al.J. Biol. Chem. 269:15668-15675 (1994) .

Background Reference 02

Van Hoof C., et al.Genomics 28:261-272 (1995) .

Background Reference 03

Janssens V., et al.Eur. J. Biochem. 267:4406-4413 (2000) .

Background Reference 04

Ota T., et al.Nat. Genet. 36:40-45 (2004) .

Other Product Names

Serine/threonine-protein phosphatase 2A activator, 5.2.1.8, PP2A, subunit B', PR53 isoform, Phosphotyrosyl phosphatase activator, PTPA, Serine/threonine-protein phosphatase 2A regulatory subunit 4, Serine/threonine-protein phosphatase 2A regulatory subunit B', PPP2R4, PTPA