NMNAT1 Antibody

Product Specifications

Background

Catalyzes the formation of NAD (+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD (+) . For the pyrophosphorolytic activity, prefers NAD (+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP (+), NADPH and NAADP (+) . Protects against axonal degeneration following mechanical or toxic insults.

NCBI Gene ID

64802

Swiss Prot

Q9HAN9

Accession Number

Q9HAN9

Host

Rabbit

Reactivity

Human

Immunogen

This NMNAT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 200-232 amino acids from human NMNAT1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Neuroscience, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

32 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

NMNAT1

NCBI Official Name

Nicotinamide mononucleotide adenylyltransferase 1

NCBI Organism

Homo sapiens

Background Reference 01

Schweiger M., et al.FEBS Lett. 492:95-100 (2001) .

Background Reference 02

Emanuelli M., et al.J. Biol. Chem. 276:406-412 (2001) .

Background Reference 03

Fernando F.S., et al.Gene 284:23-29 (2002) .

Background Reference 04

Ota T., et al.Nat. Genet. 36:40-45 (2004) .

Other Product Names

Nicotinamide mononucleotide adenylyltransferase 1, NMN adenylyltransferase 1, 2.7.7.1, Nicotinate-nucleotide adenylyltransferase 1, NaMN adenylyltransferase 1, 2.7.7.18, NMNAT1, NMNAT