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CHK1 Antibody

Product Specifications

Background

HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects. HRD1 contains one ring-type zinc finger.

NCBI Gene ID

1111

Swiss Prot

O14757

Accession Number

O14757

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from human CHK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=61-138

Calculated Molecular Weight

54 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

CHEK1

NCBI Official Name

Serine/threonine-protein kinase Chk1

NCBI Organism

Homo sapiens

Background Reference 01

Kaneko M, FEBS Lett. 2002. 532: 147-152.

Background Reference 02

Amano T, et al. Genes Dev. 2003. 17: 2436-2449.

Other Product Names

Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1

Tested Applications

WB

Physical Properties

Liquid

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