E1 Ubiquitin Antibody

Product Specifications

Background

UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.

NCBI Gene ID

7317

Swiss Prot

P22314

Accession Number

P22314

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Rabbit, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

118 kDa

Fragment

Rabbit Ig

Symbol

UBA1

NCBI Official Name

Ubiquitin-like modifier-activating enzyme 1

NCBI Organism

Homo sapiens

Background Reference 01

Ayusawa, D., et al., Cell Struct. Funct. 17 (2) :113-122 (1992) . Handley, P.M., et al., Proc. Natl. Acad. Sci. U.S.A. 88 (1) :258-262 (1991) . Kudo, M., et al., Exp. Cell Res. 192 (1) :110-117 (1991) . Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53 (1) :20-22 (1990) . Zacksenhaus, E., et al., EMBO J. 9 (9) :2923-2929 (1990) .