HIF1A Antibody
Product Specifications
Background
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.
NCBI Gene ID
3091
Swiss Prot
Q16665
Accession Number
Q16665
Host
Rabbit
Reactivity
Human
Immunogen
This HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 735-762 amino acids from the C-terminal region of human HIF1A.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Obesity
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Bovine
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
93 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
HIF1A
NCBI Official Name
Hypoxia-inducible factor 1-alpha
NCBI Organism
Homo sapiens
Background Reference 01
Gonsalves, C., et al. J. Immunol. 185 (10) :6253-6264 (2010)
Background Reference 02
Hindryckx, P., et al. J. Immunol. 185 (10) :6306-6316 (2010)
Background Reference 03
Espinosa, I., et al. Am. J. Surg. Pathol. 34 (11) :1708-1714 (2010)
Background Reference 04
Corzo, C.A., et al. J. Exp. Med. 207 (11) :2439-2453 (2010)
Other Product Names
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
Tested Applications
WB
Protein ID
2498017
Physical Properties
Liquid
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