HIF1A Antibody

Product Specifications

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

NCBI Gene ID

3091

Swiss Prot

Q16665

Accession Number

Q16665

Host

Rabbit

Reactivity

Human

Immunogen

This HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 735-762 amino acids from the C-terminal region of human HIF1A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

93 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

HIF1A

NCBI Official Name

Hypoxia-inducible factor 1-alpha

NCBI Organism

Homo sapiens

Background Reference 01

Gonsalves, C., et al. J. Immunol. 185 (10) :6253-6264 (2010)

Background Reference 02

Hindryckx, P., et al. J. Immunol. 185 (10) :6306-6316 (2010)

Background Reference 03

Espinosa, I., et al. Am. J. Surg. Pathol. 34 (11) :1708-1714 (2010)

Background Reference 04

Corzo, C.A., et al. J. Exp. Med. 207 (11) :2439-2453 (2010)

Other Product Names

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8