PUS10 Antibody

Product Specifications

Background

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]) .[supplied by OMIM].

NCBI Gene ID

150962

Swiss Prot

Q3MIT2

Accession Number

Q3MIT2

Host

Rabbit

Reactivity

Human

Immunogen

This PUS10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-78 amino acids from the N-terminal region of human PUS10.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

60 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PUS10

NCBI Official Name

Putative tRNA pseudouridine synthase Pus10

NCBI Organism

Homo sapiens

Background Reference 01

Dick, D.M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :

Background Reference 02

McGovern, D.P., et al. Nat. Genet. 42 (4) :332-337 (2010)

Background Reference 03

Dubois, P.C., et al. Nat. Genet. 42 (4) :295-302 (2010)

Background Reference 04

Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)

Other Product Names

Putative tRNA pseudouridine synthase Pus10, 5499-, Coiled-coil domain-containing protein 139, tRNA pseudouridine 55 synthase, Psi55 synthase, tRNA pseudouridylate synthase, tRNA-uridine isomerase, PUS10, CCDC139, DOBI