POLK Antibody
Product Specifications
Background
External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]) .
NCBI Gene ID
51426
Swiss Prot
Q9UBT6
Accession Number
Q9UBT6
Host
Rabbit
Reactivity
Human
Immunogen
This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
99 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
POLK
NCBI Official Name
DNA polymerase kappa
NCBI Organism
Homo sapiens
Background Reference 01
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 02
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :
Background Reference 03
Katafuchi, A., et al. Nucleic Acids Res. 38 (3) :859-867 (2010)
Background Reference 04
Fukuda, H., et al. J. Biol. Chem. 284 (38) :25585-25592 (2009)
Other Product Names
DNA polymerase kappa, DINB protein, DINP, POLK, DINB1
Tested Applications
WB
Protein ID
59798438
Physical Properties
Liquid
Frequently Asked Questions
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