USP14 Antibody

Product Specifications

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

NCBI Gene ID

9097

Swiss Prot

P54578

Accession Number

P54578

Host

Rabbit

Reactivity

Human

Immunogen

This USP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-383 amino acids from the C-terminal region of human USP14.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

56 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

USP14

NCBI Official Name

Ubiquitin carboxyl-terminal hydrolase 14

NCBI Organism

Homo sapiens

Background Reference 01

Davila, S., et al. Genes Immun. 11 (3) :232-238 (2010)

Background Reference 02

Chen, P.C., et al. J. Neurosci. 29 (35) :10909-10919 (2009)

Background Reference 03

Mines, M.A., et al. J. Biol. Chem. 284 (9) :5742-5752 (2009)

Background Reference 04

Nagai, A., et al. Biochem. Biophys. Res. Commun. 379 (4) :995-1000 (2009)

Other Product Names

Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14, USP14, TGT