PARP1 Antibody

Product Specifications

Background

This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

NCBI Gene ID

142

Swiss Prot

P09874

Accession Number

P09874

Host

Rabbit

Reactivity

Human

Immunogen

This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-618 amino acids from the Central region of human PARP1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

113 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PARP1

NCBI Official Name

Poly [ADP-ribose] polymerase 1

NCBI Organism

Homo sapiens

Background Reference 01

Majewski, P.M., et al. J. Biol. Chem. 285 (45) :34828-34838 (2010)

Background Reference 02

Kim, M., et al. Cancer Sci. 101 (11) :2436-2442 (2010)

Background Reference 03

Dong, Y., et al. Cancer Res. 70 (20) :8088-8096 (2010)

Background Reference 04

Krishnakumar, R., et al. Mol. Cell 39 (5) :736-749 (2010)

Other Product Names

Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD (+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL