NFKB1 Antibody

Product Specifications

Background

NFKB1 is a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.

NCBI Gene ID

4790

Swiss Prot

P19838

Accession Number

P19838

Host

Rabbit

Reactivity

Human

Immunogen

This NFKB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-939 amino acids from human NFKB1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Immunology, Signal Transduction, Inflammasomes

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Chicken, Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

105 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NFKB1

NCBI Official Name

Nuclear factor NF-kappa-B p105 subunit

NCBI Organism

Homo sapiens

Background Reference 01

Legembre, P., et al., J. Biol. Chem. 279 (45) :46742-46747 (2004) .

Background Reference 02

Imamura, R., et al., J. Biol. Chem. 279 (45) :46415-46423 (2004) .

Background Reference 03

Hung, J.H., et al., J. Biol. Chem. 279 (45) :46384-46392 (2004) .

Background Reference 04

Binnicker, M.J., et al., Infect. Immun. 72 (11) :6408-6417 (2004) .

Other Product Names

Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p50 subunit, NFKB1