AP2 alpha Antibody

Product Specifications

Background

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2 alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.

NCBI Gene ID

7020

Swiss Prot

P05549

Accession Number

P05549

Host

Rabbit

Reactivity

Human

Immunogen

This AP2 alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human AP2 alpha.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

48 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

TFAP2A

NCBI Official Name

Transcription factor AP-2-alpha

NCBI Organism

Homo sapiens

Background Reference 01

Provenzano, M.J., Exp. Mol. Pathol. 83 (2), 277-282 (2007)

Background Reference 02

Tan, Y.R., Biochem. J. 405 (1), 131-137 (2007)

Background Reference 03

Liu, H., EMBO Rep. (4), 394-400 (2007)

Background Reference 04

Han, S., J. Biol. Chem. 282 (11), 7961-7972 (2007)

Other Product Names

Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2, TFAP2A, AP2TF, TFAP2