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VPS25 Antibody

Product Specifications

Background

VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]) .

NCBI Gene ID

84313

Swiss Prot

Q9BRG1

Accession Number

Q9BRG1

Host

Rabbit

Reactivity

Mouse

Immunogen

This VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=60-656

Calculated Molecular Weight

21 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

VPS25

NCBI Official Name

Vacuolar protein-sorting-associated protein 25

NCBI Organism

Homo sapiens

Background Reference 01

Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

Background Reference 02

Talmud, P.J., et al. Am. J. Hum. Genet. 85 (5) :628-642 (2009)

Background Reference 03

Im, Y.J., et al. Dev. Cell 17 (2) :234-243 (2009)

Background Reference 04

Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

Other Product Names

Vacuolar protein-sorting-associated protein 25, hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, VPS25, DERP9, EAP20

Tested Applications

WB

Protein ID

73920459

Physical Properties

Liquid

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