Mouse Shc1 Antibody

Product Specifications

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p47Shc and isoform p52Shc, once phosphorylated, couple activated receptor kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p47Shc and isoform p52 may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.

NCBI Gene ID

20416

Swiss Prot

P98083

Accession Number

P98083

Host

Rabbit

Reactivity

Mouse

Immunogen

This Mouse Shc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-405 amino acids from the Central region of mouse Shc1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

63 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

Shc1

NCBI Official Name

SHC-transforming protein 1

NCBI Organism

Mus musculus

Background Reference 01

Ursini-Siegel, J., et al. Cancer Res. 70 (20) :7776-7787 (2010)

Background Reference 02

Ma, Z., et al. Oncogene 29 (41) :5559-5567 (2010)

Background Reference 03

Fadini, G.P., et al. Diabetes 59 (9) :2306-2314 (2010)

Background Reference 04

Ranieri, S.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (30) :13420-13425 (2010)

Other Product Names

SHC-transforming protein 1, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1, Shc1, Shc, ShcA