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EIF4E Antibody

Product Specifications

Background

EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

NCBI Gene ID

1977

Swiss Prot

P06730

Accession Number

P06730

Host

Rabbit

Reactivity

Human

Immunogen

This EIF4E antibody is generated from rabbits immunized with a recombinant protein from human VGFR2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=60-601

Calculated Molecular Weight

25 kDa

Fragment

Rabbit Ig

Symbol

EIF4E

NCBI Official Name

Eukaryotic translation initiation factor 4E

NCBI Organism

Homo sapiens

Background Reference 01

Kim, S., et.al., J. Virol. 84 (1), 52-58 (2010)

Background Reference 02

Kao, C.L., et.al., Cancer Lett. 286 (2), 250-259 (2009)

Other Product Names

Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F

Tested Applications

WB, IHC-P, Flow

Protein ID

1352435

Physical Properties

Liquid

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