HERC3 Antibody

Product Specifications

Background

HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710) -like domain (RLD) . The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]) .

NCBI Gene ID

8916

Swiss Prot

Q15034

Accession Number

Q15034

Host

Rabbit

Reactivity

Human

Immunogen

This HERC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human HERC3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

117 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

HERC3

NCBI Official Name

Probable E3 ubiquitin-protein ligase HERC3

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 02

Hochrainer, K., et al. Genomics 85 (2) :153-164 (2005)

Background Reference 03

Cruz, C., et al. FEBS Lett. 488 (1-2), 74-80 (2001) :

Background Reference 04

Cruz, C., et al. Cytogenet. Cell Genet. 87 (3-4), 263-264 (1999) :

Other Product Names

Probable E3 ubiquitin-protein ligase HERC3, 632-, HECT domain and RCC1-like domain-containing protein 3, HERC3, KIAA0032

Tested Applications

Protein ID

2495699

Physical Properties

Liquid

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