SF3B4 Antibody

Product Specifications

Background

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

NCBI Gene ID

10262

Swiss Prot

Q15427

Accession Number

Q15427

Host

Rabbit

Reactivity

Human

Immunogen

This SF3B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-187 amino acids from the Central region of human SF3B4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

44 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

SF3B4

NCBI Official Name

Splicing factor 3B subunit 4

NCBI Organism

Homo sapiens

Background Reference 01

Gudbjartsson, D.F., et al. Nat. Genet. 40 (5) :609-615 (2008)

Background Reference 02

Rikova, K., et al. Cell 131 (6) :1190-1203 (2007)

Background Reference 03

Watanabe, H., et al. J. Biol. Chem. 282 (28) :20728-20738 (2007)

Background Reference 04

Wu, C., et al. Proteomics 7 (11) :1775-1785 (2007)

Other Product Names

Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SF3b50, Spliceosome-associated protein 49, SAP 49, SF3B4, SAP49

Tested Applications

Protein ID

2500587

Physical Properties

Liquid

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