MBD4 Antibody
Product Specifications
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.
NCBI Gene ID
8930
Swiss Prot
O95243
Accession Number
O95243
Host
Rabbit
Reactivity
Human
Immunogen
This MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the Central region of human MBD4.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=60-515
Calculated Molecular Weight
66 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
MBD4
NCBI Official Name
Methyl-CpG-binding domain protein 4
NCBI Organism
Homo sapiens
Background Reference 01
Arora, M., et al. Leukemia 24 (8) :1470-1475 (2010)
Background Reference 02
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16 (8) :1084-1089 (2010)
Background Reference 03
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
Background Reference 04
Briggs, F.B., et al. Am. J. Epidemiol. 172 (2) :217-224 (2010)
Other Product Names
Methyl-CpG-binding domain protein 4, 322-, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, MBD4, MED1
Tested Applications
WB
Physical Properties
Liquid
Curated Selection
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