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RFC1 Antibody

Product Specifications

Background

The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq].

NCBI Gene ID

5981

Swiss Prot

P35251

Accession Number

P35251

Host

Rabbit

Reactivity

Human

Immunogen

This RFC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human RFC1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

128 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

RFC1

NCBI Official Name

Replication factor C subunit 1

NCBI Organism

Homo sapiens

Background Reference 01

Overmeer, R.M., et al. Mol. Cell. Biol. 30 (20) :4828-4839 (2010) Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010) Arora, M., et al. Leukemia 24 (8) :1470-1475 (2010) Galbiatti, A.L., et al. Mol. Biol. Rep. (2010) In pressJugessur, A., et al. PLoS ONE (7), E11493 (2010) :

Other Product Names

Replication factor C subunit 1, Activator 1 140 kDa subunit, A1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA-binding protein PO-GA, Replication factor C 140 kDa subunit, RF-C 140 kDa subunit, RFC140, Replication factor C large subunit, RFC1, RFC140

Tested Applications

WB

Protein ID

56757608

Physical Properties

Liquid

Frequently Asked Questions

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