RENT1 Antibody

Product Specifications

Background

RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD) . When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.

NCBI Gene ID

5976

Swiss Prot

Q92900

Accession Number

Q92900

Host

Rabbit

Reactivity

Human

Immunogen

This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583-612 amino acids from the Central region of human RENT1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

124 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

UPF1

NCBI Official Name

Regulator of nonsense transcripts 1

NCBI Organism

Homo sapiens

Background Reference 01

Ohnishi, T., et al., Mol. Cell 12 (5) :1187-1200 (2003) . Lykke-Andersen, J., Mol. Cell. Biol. 22 (23) :8114-8121 (2002) . Carastro, L.M., et al., Nucleic Acids Res. 30 (10) :2232-2243 (2002) . Mendell, J.T., et al., Science 298 (5592) :419-422 (2002) . Serin, G., et al., Mol. Cell. Biol. 21 (1) :209-223 (2001) .

Other Product Names

Regulator of nonsense transcripts 1, 364-, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1, UPF1, KIAA0221, RENT1