Mouse Wee1 Antibody

Product Specifications

Background

Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity) .

NCBI Gene ID

22390

Swiss Prot

P47810

Accession Number

P47810

Host

Rabbit

Reactivity

Human

Immunogen

This Mouse Wee1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of mouse Wee1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

72 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

Wee1

NCBI Official Name

Wee1-like protein kinase

NCBI Organism

Mus musculus

Background Reference 01

Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010) :

Background Reference 02

Kim, M.J., et al. Oncol. Rep. 19 (5) :1323-1329 (2008)

Background Reference 03

Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352 (1) :21-28 (2007)

Background Reference 04

Tominaga, Y., et al. Int. J. Biol. Sci. 2 (4) :161-170 (2006)

Other Product Names

Wee1-like protein kinase, Wee1A kinase, Wee1

Tested Applications

Physical Properties

Liquid

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