FZR1 Antibody

Product Specifications

Background

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

NCBI Gene ID

51343

Swiss Prot

Q9UM11

Accession Number

Q9UM11

Host

Rabbit

Reactivity

Human

Immunogen

This FZR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-81 amino acids from the N-terminal region of human FZR1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

55 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

FZR1

NCBI Official Name

Fizzy-related protein homolog

NCBI Organism

Homo sapiens

Background Reference 01

Olson, J.E., et al. Breast Cancer Res. Treat. 125 (1) :221-228 (2011)

Background Reference 02

Colombo, S.L., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (44) :18868-18873 (2010)

Background Reference 03

Naoe, H., et al. Mol. Cell. Biol. 30 (16) :3994-4005 (2010)

Background Reference 04

Sigl, R., et al. J. Cell. Sci. 122 (PT 22), 4208-4217 (2009) :

Other Product Names

Fizzy-related protein homolog, Fzr, CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1, FZR1, CDH1, FYR, FZR, KIAA1242

Tested Applications

Protein ID

37537753

Physical Properties

Liquid

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