NR1H4 Antibody

Product Specifications

Background

NR1H4 is a Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP) . Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.

NCBI Gene ID

9971

Swiss Prot

Q96RI1

Accession Number

Q96RI1

Host

Rabbit

Reactivity

Human

Immunogen

This NR1H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human NR1H4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

56 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NR1H4

NCBI Official Name

Bile acid receptor

NCBI Organism

Homo sapiens

Background Reference 01

Meyer Zu Schwabedissen, H.E., et al. Hepatology 52 (5) :1797-1807 (2010)

Background Reference 02

Popescu, I.R., et al. FEBS Lett. 584 (13) :2845-2851 (2010)

Background Reference 03

Gadaleta, R.M., et al. Biochim. Biophys. Acta 1801 (7) :683-692 (2010)

Background Reference 04

Roberts, K.E., et al. Gastroenterology 139 (1) :130-139 (2010)

Other Product Names

Bile acid receptor, Farnesoid X-activated receptor, Farnesol receptor HRR-1, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14, RXR-interacting protein 14, NR1H4, BAR, FXR, HRR1, RIP14