POLR1A Antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity) .

This POLR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Rose, J. Phd, et al. Mol. Med. (2010) In pressPercipalle, P., et al. EMBO Rep. 7 (5) :525-530 (2006) Bouwmeester, T., et al. Nat. Cell Biol. 6 (2) :97-105 (2004) Hirschler-Laszkiewicz, I., et al. J. Biol. Chem. 278 (21) :18953-18959 (2003) Dundr, M., et al. Science 298 (5598) :1623-1626 (2002)

DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194 kDa subunit, RPA194, POLR1A